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Evident Corporation 31000v2 filter cube dapi
Cultures of SEA007 and SEA008 were grown as in . Samples were taken approximately 2.5 hrs. after induction of rseA and rseB overexpression. (A–D) Images of live cells are shown using differential interference microscopy (DIC, column 1) and fluorescence microscopy following addition of FM4-64 to visualize membranes (red, column 2), <t>DAPI</t> to visualize DNA (blue, column 3), and expression <t>of</t> <t>YFP</t> to visualize the cytoplasm (green, column 4). The three fluorescent micrographs are overlaid in column 5. (A) Images of the SEA008 control strain in which σ E was not inhibited are shown. (B–D) Images of SEA007 following σ E inhibition, reveal blebs that contain YFP and stain with DAPI (B and C) and that contain YFP but do not stain with DAPI (D). In (D), the arrow marks a bleb lacking DAPI staining and the arrowhead marks a lysed cell that retained DAPI staining, but lost YFP. Scale bars are 2 µm. Over 1,000 cells were examined by fluorescence microscopy and typical micrographs are represented here. Scanning electron micrographs (E) and transmission electron micrographs (F) of SEA007 following σ E inhibition. Scale bars are 1 µm. No blebs were seen on cells in control cultures.
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Chroma Technology Corporation triple filter set no. 61000v2 bs&m
Cultures of SEA007 and SEA008 were grown as in . Samples were taken approximately 2.5 hrs. after induction of rseA and rseB overexpression. (A–D) Images of live cells are shown using differential interference microscopy (DIC, column 1) and fluorescence microscopy following addition of FM4-64 to visualize membranes (red, column 2), <t>DAPI</t> to visualize DNA (blue, column 3), and expression <t>of</t> <t>YFP</t> to visualize the cytoplasm (green, column 4). The three fluorescent micrographs are overlaid in column 5. (A) Images of the SEA008 control strain in which σ E was not inhibited are shown. (B–D) Images of SEA007 following σ E inhibition, reveal blebs that contain YFP and stain with DAPI (B and C) and that contain YFP but do not stain with DAPI (D). In (D), the arrow marks a bleb lacking DAPI staining and the arrowhead marks a lysed cell that retained DAPI staining, but lost YFP. Scale bars are 2 µm. Over 1,000 cells were examined by fluorescence microscopy and typical micrographs are represented here. Scanning electron micrographs (E) and transmission electron micrographs (F) of SEA007 following σ E inhibition. Scale bars are 1 µm. No blebs were seen on cells in control cultures.
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Sutter Instrument Company sutter instruments lambda ls light source with dapi long pass filter set
Cultures of SEA007 and SEA008 were grown as in . Samples were taken approximately 2.5 hrs. after induction of rseA and rseB overexpression. (A–D) Images of live cells are shown using differential interference microscopy (DIC, column 1) and fluorescence microscopy following addition of FM4-64 to visualize membranes (red, column 2), <t>DAPI</t> to visualize DNA (blue, column 3), and expression <t>of</t> <t>YFP</t> to visualize the cytoplasm (green, column 4). The three fluorescent micrographs are overlaid in column 5. (A) Images of the SEA008 control strain in which σ E was not inhibited are shown. (B–D) Images of SEA007 following σ E inhibition, reveal blebs that contain YFP and stain with DAPI (B and C) and that contain YFP but do not stain with DAPI (D). In (D), the arrow marks a bleb lacking DAPI staining and the arrowhead marks a lysed cell that retained DAPI staining, but lost YFP. Scale bars are 2 µm. Over 1,000 cells were examined by fluorescence microscopy and typical micrographs are represented here. Scanning electron micrographs (E) and transmission electron micrographs (F) of SEA007 following σ E inhibition. Scale bars are 1 µm. No blebs were seen on cells in control cultures.
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IDEX dapi/cy5 (custom 239415) dual emission filters
An overview of the reagents used for immunohistochemistry
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Chroma Technology Corporation dapi filter
An overview of the reagents used for immunohistochemistry
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Image Search Results


Cultures of SEA007 and SEA008 were grown as in . Samples were taken approximately 2.5 hrs. after induction of rseA and rseB overexpression. (A–D) Images of live cells are shown using differential interference microscopy (DIC, column 1) and fluorescence microscopy following addition of FM4-64 to visualize membranes (red, column 2), DAPI to visualize DNA (blue, column 3), and expression of YFP to visualize the cytoplasm (green, column 4). The three fluorescent micrographs are overlaid in column 5. (A) Images of the SEA008 control strain in which σ E was not inhibited are shown. (B–D) Images of SEA007 following σ E inhibition, reveal blebs that contain YFP and stain with DAPI (B and C) and that contain YFP but do not stain with DAPI (D). In (D), the arrow marks a bleb lacking DAPI staining and the arrowhead marks a lysed cell that retained DAPI staining, but lost YFP. Scale bars are 2 µm. Over 1,000 cells were examined by fluorescence microscopy and typical micrographs are represented here. Scanning electron micrographs (E) and transmission electron micrographs (F) of SEA007 following σ E inhibition. Scale bars are 1 µm. No blebs were seen on cells in control cultures.

Journal: PLoS ONE

Article Title: The Extracytoplasmic Stress Factor, σ E , Is Required to Maintain Cell Envelope Integrity in Escherichia coli

doi: 10.1371/journal.pone.0001573

Figure Lengend Snippet: Cultures of SEA007 and SEA008 were grown as in . Samples were taken approximately 2.5 hrs. after induction of rseA and rseB overexpression. (A–D) Images of live cells are shown using differential interference microscopy (DIC, column 1) and fluorescence microscopy following addition of FM4-64 to visualize membranes (red, column 2), DAPI to visualize DNA (blue, column 3), and expression of YFP to visualize the cytoplasm (green, column 4). The three fluorescent micrographs are overlaid in column 5. (A) Images of the SEA008 control strain in which σ E was not inhibited are shown. (B–D) Images of SEA007 following σ E inhibition, reveal blebs that contain YFP and stain with DAPI (B and C) and that contain YFP but do not stain with DAPI (D). In (D), the arrow marks a bleb lacking DAPI staining and the arrowhead marks a lysed cell that retained DAPI staining, but lost YFP. Scale bars are 2 µm. Over 1,000 cells were examined by fluorescence microscopy and typical micrographs are represented here. Scanning electron micrographs (E) and transmission electron micrographs (F) of SEA007 following σ E inhibition. Scale bars are 1 µm. No blebs were seen on cells in control cultures.

Article Snippet: Cells were mounted on freshly prepared polylysine coated coverslips and visualized using an Olympus BX-61 epi-fluorescent microscope with a 100× phase oil objective (NA of 1.3), using an Olympus 41028 filter cube for YFP, Olympus 31000v2 filter cube for DAPI, and an Olympus 41001 filter cube for FM4-64.

Techniques: Over Expression, Microscopy, Fluorescence, Expressing, Inhibition, Staining, Transmission Assay

An overview of the reagents used for immunohistochemistry

Journal: The Journal of Neuroscience

Article Title: Cholinergic Control of GnRH Neuron Physiology and Luteinizing Hormone Secretion in Male Mice: Involvement of ACh/GABA Cotransmission

doi: 10.1523/JNEUROSCI.1780-23.2024

Figure Lengend Snippet: An overview of the reagents used for immunohistochemistry

Article Snippet: At the same time, to detect Cy5 emission, a He–Ne laser (637 nm) and a DAPI/Cy5 (custom 239415; Semrock) dual emission filters were applied.

Techniques: Immuno-Electron Microscopy, Electron Microscopy